ABSTRACT
Objective To explore the expression characteristics of cytochrome P450(CYP)candidate genes(CYP6M3, CYP6Y1,CYP6P5,CYP4H14,CYP4G17,CYP12F16)in Anopheles sinensis.Methods The samples were collected from dif-ferent developmental stages(eggs,larvae,pupae and adult mosquitoes(females and males)),and different tissues(salivary glands,malpighian tubes,midguts,ovaries,and fat bodies)of An.sinensis and the female adult mosquitoes exposed by differ-ent insecticide doses(0,1.25,3.75,6.25,12.5μg/bottle)and time points(0,5,15,30,60 minutes),then the total RNA was extracted. Quantitative reverse transcription PCR(qPCR)assay was used to analyze the relative expressions of six CYP genes in An.sinensis at different developmental stages,tissues and different insecticide exposure doses and time.Results The expressions of CYP6M3 and CYP6Y1 in the male adult mosquitoes were the highest,the expression of CYP6M3 gene in the males was 35.1 times higher than that in the females,the expression of CYP6Y1 in the males was 61.4 times higher than that in the females;the expression level of CYP4H14 in the larvae was the lowest,the expression of CYP4H14 in the females was 22.5 times higher than that in the fourth instar larvae.The expressions of candidate CYP genes in different tissues of An.sinensis were significantly different,the expression of CYP6M3 in the malpighian tubule was 38.9 times higher than that in the ovary,the ex-pression of CYP6Y1 in the fatbody was 9.1 times higher than that in the ovary,the expression of CYP4G17 was 4.6 times higher than that in the ovary,and the expression of CYP12F16 was 4.4 times higher thanthat in the ovary.The exposure to different in-secticide doses and time showed some induction effects in the expressions of candidate CYP genes,which affected the expres-sions of candidate CYP genes in An.sinensis.Conclusion The expression of candidate CYP genes varies significantly in differ-ent developmental stages and various tissues of An.sinensis,and exposure to deltamethrin at various doses and time points af-fects CYP genes expression in An.sinensis.
ABSTRACT
Objective: To investigate the effect of cold stress on the three gene families (PgHMGR, PgSSs, and PgSEs) of the ginsenoside biosynthetic pathway, and to explore the mechanism of genes response to cold stress and find the key genes. Methods: The cold stress treatment was performed by selecting 3 weeks fresh callus which placed in the 5℃ refrigerator, and harvested after 0, 0.5, 1, 2, and 3 d treatment for further analysis, were recorded as CK, D1, D2, D3, and D4, respectively. Results: The expression of PgHMGR1 reached 1.3 times compared to the control group at D3 period, and the expression of PgHMGR2 reached the peak at D1 period, which was 3.8 times of the control group; the expression of PgSS1 was 1.7 times of the control group (D3 period); and the expressions of PgSE2 and PgSE1 were 6.9 and 6 times higher than those of the control group, respectively. There was no significant change of the expression of PgSS2, PgSE3, and PgHMGR3.Conclusion: The gene families of ginsenoside biosynthesis pathway positively response to cold stress treatment, and PgHMGR1, PgHMGR2, PgSS1, PgSE1, and PgSE2 may be the key family genes when Panax ginseng callus response to cold stress.